Description
The ViPrimePLUS Hepatitis E Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Hepatitis E Virus genome in faecal matter and food or water sample.
Hepatitis E virus is a single stranded RNA virus with a linear genome. There are about 20 million new cases reported each year globally. Transmission is usually through contaminated drinking water, i.e. fecal-oral route. HEV infection is normally self-limiting without lasting damage. However in rare occasion, fulminant hepatitis may develop leading to acute liver failure, with higher prevalence among pregnant ladies. Re-activation of HEV is found in immunocompromised patients, resulting in chronic infection.
ViPrimePLUS Hepatitis E Virus RT-qPCR Kit is intended for research use only.
Kit Component
- HEV Primers and Probe Mix (HEV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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• Clinical samples (e.g. faecal)
• Food samples
• Water samples
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Gene Target
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Open reading frame 2 (ORF2) region
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM5014
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ViPrimePLUS Hepatitis E Virus RT-qPCR Kit
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150 reactions
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