Description
The ViPrimePLUS Hepatitis C Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Hepatitis C Virus genome in clinical samples (e.g. blood plasma, serum and liver biopsy).
Hepatitis C virus is a small, enveloped, single stranded RNA virus. About 3-4million people worldwide are infected with the virus each year and 350 000 deaths resulted from it. The virus is transmitted through contact with infected blood. Hepatitis C causes both acute and chronic infection which leads to liver cirrhosis, liver cancer and fatal liver failure. Because of its severity, Hepatitis C disease is the main reason for liver transplants in US. Vaccines have yet to be developed due to the highly mutable RNA genome. Anti-viral drug are currently in the stages of clinical studies. Thus, fast and accurate detection is essential in preventing the irreversible liver damage.
ViPrimePLUS Hepatitis C Virus RT-qPCR Kit is intended for research use only.
Kit Component
- HCV Primers and Probe Mix (HCV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. blood plasma, serum, liver biopsy)
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Gene Target
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5’ untranslated region
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM5012
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ViPrimePLUS Hepatitis C Virus RT-qPCR Kit
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150 reactions
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